Title :
A nonlinear approach to tracking single nanometer-scale fluorescent particles
Author_Institution :
Dept. of Mech. Eng., Boston Univ., Boston, MA, USA
fDate :
June 30 2010-July 2 2010
Abstract :
We describe a nonlinear control law for tracking single, sub-diffraction limit fluorescent particles that takes advantage of the geometric properties of the spatial intensity pattern. We present simulation results for tracking a single particle based on confocal measurements to illustrate the approach. The control law converts each intensity measurement into actuator commands directly, and consequently should yield improved temporal response over existing algorithms. We consider both two- and three-dimensional tracking.
Keywords :
biology; fluorescence; geometry; molecular biophysics; nanoparticles; nonlinear control systems; actuator commands; confocal measurements; geometric property; intensity measurement; nonlinear control law; single nanometer-scale fluorescent particle tracking; spatial intensity pattern; subdiffraction limit fluorescent particle; three-dimensional tracking; two-dimensional tracking; Detectors; Fluorescence; Focusing; Image converters; Magnetic field measurement; Microscopy; Nanobioscience; Particle measurements; Particle tracking; Position measurement;
Conference_Titel :
American Control Conference (ACC), 2010
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4244-7426-4
DOI :
10.1109/ACC.2010.5530946
