Title :
A novel microchip system integrated with gold nano-electrode ensemble for electrochemical determination of hyaluronic acid
Author :
Chen, Chun-Mao ; Yeh, Ming-Long ; Chuang, Ya-Ting ; Lin, Che-Hsin ; Chien, Chi-Sheng
Author_Institution :
Inst. of Biomed. Eng., Nat. Cheng Kung Univ., Tainan, Taiwan
Abstract :
This paper proposes an innovative microfluidic device for hyaluronic acid (HA) extraction and electrochemical detection utilizing PMMA based microchip that integrated with gold nanoelectrode ensemble (GNEE). Gold nanoelectrode ensemble is fabricated using electroless deposition in a thin polycarbonate (PC) film and then directly bonded onto the PMMA substrate for high performance electrochemical detection. In the study, microwave is firstly used to decrease the interference of protein on the working electrode, and then electrostatic force is used to separate and extract HA in the rat serum sample. The used of GNEE electrodes in the microchip not only enhances the signal but also decreases the background noises, resulting a high detection limit of 0.1 mg/mL for HA, and showing good linear responses in the concentration range of 10 mg/mL-0.1 mg/mL (R2=0.956). The proposed microchip device provides a fast detection method for hyaluronic acid analysis.
Keywords :
electroless deposition; gold; microfluidics; proteins; Au; GNEE electrodes; PMMA based microchip; PMMA substrate; electrochemical determination; electroless deposition; electrostatic force; gold nano-electrode ensemble; high performance electrochemical detection; hyaluronic acid; microchip system; microfluidic device; protein; rat serum sample; thin polycarbonate film; working electrode; Background noise; Bonding; Electrodes; Electrostatic interference; Gold; Microfluidics; Microwave devices; Nanoscale devices; Proteins; Substrates;
Conference_Titel :
Sensors, 2009 IEEE
Conference_Location :
Christchurch
Print_ISBN :
978-1-4244-4548-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2009.5398450