Characterizing strain in an embryonic development inspired method for engineered tendon

We have developed a scaffold-free, cell-based, single fiber approach toward tissue engineered tendon. This method provides a culture system in which key aspects of embryonic tenogenesis may be replicated, such as high initial cell density, cell-cell adhesion, and dynamic mechanical cues. Through the application of embryonic development inspired growth factors and mechanical stimulation, we ultimately hope to create a mechanically and structurally matched tendon replacement. We demonstrate here, the ability to apply a well characterized cyclic tensile strain to cell-based, single fibers during development.