DocumentCode
3318218
Title
Notice of Retraction
Expression of Functional Recombinant Barnacle Cement Protein Bacp-20k in Escherichia coli.
Author
Yanan Sun ; Lina Du ; Zhen Jiang ; Xiyu Ding ; Hui Xu ; Yabiao Yu ; Qiqing Zhang
Author_Institution
Dept. of Biomater., Xiamen Univ., Xiamen, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The barnacle is a unique sessile crustacean, which produces an insoluble proteinaceous cement for its attachment to underwater foreign substrata. The 20 kDa cement protein is a component of Balanus albicostatus cement. We amplified Bacp-20k gene from the basal tissue and cloned the target gene into the prokaryotic expressing vector, pET-32a. After transformation, a soluble protein fused with a hexahistidine affinity ligand was expressed in E.coli Rosettagami (DE3). The recombinant protein was highly purified using affinity chromatography. The adhesive properties of purified recombinant Bacp-20k were examined by quartz crystal microbalance. The results indicated that recombinant Bacp-20k had strong adhesive ability and showed the possibility to be a practical water-resistant bioadhesive in medical or underwater environments.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The barnacle is a unique sessile crustacean, which produces an insoluble proteinaceous cement for its attachment to underwater foreign substrata. The 20 kDa cement protein is a component of Balanus albicostatus cement. We amplified Bacp-20k gene from the basal tissue and cloned the target gene into the prokaryotic expressing vector, pET-32a. After transformation, a soluble protein fused with a hexahistidine affinity ligand was expressed in E.coli Rosettagami (DE3). The recombinant protein was highly purified using affinity chromatography. The adhesive properties of purified recombinant Bacp-20k were examined by quartz crystal microbalance. The results indicated that recombinant Bacp-20k had strong adhesive ability and showed the possibility to be a practical water-resistant bioadhesive in medical or underwater environments.
Keywords
adhesives; biological tissues; biomechanics; biomedical materials; cellular biophysics; chromatography; crystal purification; genetics; microbalances; microorganisms; molecular biophysics; proteins; Bacp-20k gene; Balanus albicostatus cement; E.coli Rosettagami; Escherichia coli; adhesive properties; affinity chromatography; basal tissue; cloning; functional recombinant barnacle cement protein Bacp; hexahistidine affinity ligand; medical environment; pET-32a; prokaryotic expressing vector; proteinaceous cement; purification; quartz crystal microbalance; sessile crustacean; target gene; underwater environment; underwater foreign substrata; water-resistant bioadhesive; Adhesives; Cloning; Crystals; Gold; Protein engineering; Proteins; USA Councils;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780054
Filename
5780054
Link To Document