DocumentCode
3322547
Title
Notice of Retraction
Characterization of Salsolinol Synthase from Rat Brain
Author
Xuechai Chen ; Arshad, A. ; Yi Chen ; Shengping Dai ; Shengyuan Xiao ; Yulin Deng
Author_Institution
Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Several studies estimating the function of salsolinol (1 methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline), a dopamine-derived endogenously synthesized compound, have revealed its involvement in the pathogenesis of neurodegenerative diseases such as Parkinson´s disease (PD). Moreover, a number of investigations propose that N-methylated derivates of salsolinol, N-methyl-salsolinol and its metabolite products are also involved in etiopathogenesis of PD. The human brain has a higher concentration of (R) enantiomer of salsolinol than (S)enantiomer, suggesting its enzymatic biosynthesis. Recent studies in our lab have shown salsolinol synthesized from dopamine and acetaldehyde by a novel enzyme called salsolinol synthase. In current investigations, the brains of male Sprague Dawley rats were used for crude extract isolation and HPLC-electrochemical detection (ECD) was used for the activity assay of this putative enzyme. Our results show the enzyme activity of salsolinol synthase to be maximum at 80°C and at pH of 7. The results also showed that amount of Salsolinol synthesized by salsolinol synthase is directly proportional to the amount of substrates dopamine (DA) and acetaldehyde (Ach) at 37°C. Its isoelectric point was 8.0 determined with Off-gel. Further characterization of this putative enzyme is under process which will highlight more profound knowledge about this putative enzyme and hence, will help elucidate the pathogenesis of Parkinson´s like diseases and will provide a strong basis for- treatment of such neurodegenerative diseases.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Several studies estimating the function of salsolinol (1 methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline), a dopamine-derived endogenously synthesized compound, have revealed its involvement in the pathogenesis of neurodegenerative diseases such as Parkinson´s disease (PD). Moreover, a number of investigations propose that N-methylated derivates of salsolinol, N-methyl-salsolinol and its metabolite products are also involved in etiopathogenesis of PD. The human brain has a higher concentration of (R) enantiomer of salsolinol than (S)enantiomer, suggesting its enzymatic biosynthesis. Recent studies in our lab have shown salsolinol synthesized from dopamine and acetaldehyde by a novel enzyme called salsolinol synthase. In current investigations, the brains of male Sprague Dawley rats were used for crude extract isolation and HPLC-electrochemical detection (ECD) was used for the activity assay of this putative enzyme. Our results show the enzyme activity of salsolinol synthase to be maximum at 80°C and at pH of 7. The results also showed that amount of Salsolinol synthesized by salsolinol synthase is directly proportional to the amount of substrates dopamine (DA) and acetaldehyde (Ach) at 37°C. Its isoelectric point was 8.0 determined with Off-gel. Further characterization of this putative enzyme is under process which will highlight more profound knowledge about this putative enzyme and hence, will help elucidate the pathogenesis of Parkinson´s like diseases and will provide a strong basis for- treatment of such neurodegenerative diseases.
Keywords
brain; diseases; enzymes; neurophysiology; pH; 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline; Parkinson´s disease; Sprague Dawley rats; acetaldehyde; dopamine; enantiomer; enzymatic biosynthesis; etiopathogenesis; isoelectric point; neurodegenerative disease; pH 7; rat brain; salsolinol synthase; temperature 37 degC; temperature 80 degC; Biochemistry; Chemicals; Diseases; Humans; Purification; Rats; Substrates;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780301
Filename
5780301
Link To Document