Notice of RetractionROS and Ca²+ Signal Activator-Resveratrol Induce Human Hepatoma Cancer Cell HepG-2 Apoptosis

Notice of Retraction

After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.

We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.

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To study mechanism of resveratrol induce HepG-2 apoptosis via Ca²⁺ and ROS pathway. Measurements using mononuclear cell direct cytotoxicity assay(the MTT method) shows that its cytotoxic effect on HepG-2 is strong with IC₅₀ being 34.86 μmol·L^-1. Measurement of action of apoptosis using morphology show that karyoplasmic ratio of tumor cell become diminish, Cells break up into several apoptotic bodies of different sizes. Measurement rate of apoptosis and cell cycle dealed with RES using flow cytometry shows that RES leads to a decrease in the proportion of cells in G₀/G₁ phase, an increasement in proportion of cells in S phase and decrease in proportion of cells in G₂/M phase. Rates of apoptosis induced by RES are 26.130%, 31.599%, 65.655%. Measurement Ca²⁺ content in HepG-2 treated with RES using laser scanning confocal microscope with Fluo-3 stain shows that RES can obviously increase Ca²⁺ content. Measurement content of ROS in HepG-2 treated with RES shows that RES can enhance the content of ROS. RES can induce HepG-2 apoptosis and activate Ca²⁺ and ROS signaling pathway.