Notice of RetractionExpression and Purification of Non-Structural Protein NS1 of Human Bocavirus and Generation of Its Antibody

Notice of Retraction

After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.

We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.

The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.

Human bocavirus (HBoV) was first discovered in 2005 in nasopharyngeal aspirates of children with respiratory tract infection. To further investigate functions of NS1 of the HBoV, we need badly the NS1 antibody, therefore, we used a prokaryotic expression system pMAL-c2x vector for the production of NS1 as a fusion protein with maltose binding protein (MBP). The target fusion protein which exhibited a strong reaction with anti-MBP antibody was purified by Amylose affinity chromatography to immunize the New Zealand white rabbit for preparing the corresponding antibody. The titer of the produced antibody was measured by ELISA. It was found by using Western blot analysis that polyclonal antibodies were specific against both the NS1 and MBP.