Notice of RetractionEffect of Oxytetracycline on Two Enzymes in CYP450 2B Subfamily in Earthworm

Notice of Retraction

After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.

We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.

The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.

Oxytetracycline (OTC) is a normal used antibiotic in animal breeding and human clinic. There were large residues of this antibiotic discharged into the environment with the waste and manure. However, almost no study has concerned its effect on the enzymatic activities in earthworms. In present study, we investigated the activities of two important enzymes in cytochrome P450 2B subfamily: pentoxy resorufin -O dealkylase (PROD), benzoxyresorufin-O -dealkylase (BROD) in earthworm Eisenia andrei exposed to OTC. 4 different concentration groups of OTC were designed to simulate the process that OTC was discharged into soil with animal manure. BROD, PROD activities in worms were respectively measured in different exposure time. The results showed that the two enzymes in worms were inhibited by OTC in high contamination level, but not in low level. The inhibition showed time or dose dependent to some extent. The further study is necessary to indentify if BROD, PROD might be used as biomarker of OTC contamination.