DocumentCode
3343383
Title
Notice of Retraction
Effect of Oxytetracycline on Two Enzymes in CYP450 2B Subfamily in Earthworm
Author
Yinsheng Li ; Xiuhong Wang ; Jiangping Qiu
Author_Institution
Sch. of Agric. & Biol., Shanghai Jiao Tong Univ., Shanghai, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
3
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Oxytetracycline (OTC) is a normal used antibiotic in animal breeding and human clinic. There were large residues of this antibiotic discharged into the environment with the waste and manure. However, almost no study has concerned its effect on the enzymatic activities in earthworms. In present study, we investigated the activities of two important enzymes in cytochrome P450 2B subfamily: pentoxy resorufin -O dealkylase (PROD), benzoxyresorufin-O -dealkylase (BROD) in earthworm Eisenia andrei exposed to OTC. 4 different concentration groups of OTC were designed to simulate the process that OTC was discharged into soil with animal manure. BROD, PROD activities in worms were respectively measured in different exposure time. The results showed that the two enzymes in worms were inhibited by OTC in high contamination level, but not in low level. The inhibition showed time or dose dependent to some extent. The further study is necessary to indentify if BROD, PROD might be used as biomarker of OTC contamination.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Oxytetracycline (OTC) is a normal used antibiotic in animal breeding and human clinic. There were large residues of this antibiotic discharged into the environment with the waste and manure. However, almost no study has concerned its effect on the enzymatic activities in earthworms. In present study, we investigated the activities of two important enzymes in cytochrome P450 2B subfamily: pentoxy resorufin -O dealkylase (PROD), benzoxyresorufin-O -dealkylase (BROD) in earthworm Eisenia andrei exposed to OTC. 4 different concentration groups of OTC were designed to simulate the process that OTC was discharged into soil with animal manure. BROD, PROD activities in worms were respectively measured in different exposure time. The results showed that the two enzymes in worms were inhibited by OTC in high contamination level, but not in low level. The inhibition showed time or dose dependent to some extent. The further study is necessary to indentify if BROD, PROD might be used as biomarker of OTC contamination.
Keywords
antibacterial activity; biochemistry; biological techniques; chemical hazards; enzymes; inhibitors; molecular biophysics; soil pollution; toxicology; CYP450 2B; animal breeding; antibiotics; benzoxyresorufin-O-dealkylase; cytochrome P450; earthworm Eisenia andrei; enzymes; human clinic product; manure; oxytetracycline effect; pentoxy resorufin-O-dealkylase; soil pollution; waste; Animals; Antibiotics; Biochemistry; Contamination; Grippers; Humans; Soil;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5781401
Filename
5781401
Link To Document