Coherent anti-Stokes Raman scattering microscopy

Author

Holtom, G.R. ; Zumbusch, A. ; Sunney Xie, X.

Author_Institution

William R. Wiley Environ. Molecular Sci. Lab., Pacific Northwest Lab., Richland, WA, USA

fYear

1992

fDate

23-28 May 1992

Firstpage

33

Lastpage

34

Abstract

Summary form only given. We report a new nonlinear optical method for high resolution microscopy which is based on a well established technique, Coherent Anti-Stokes Raman Spectroscopy (CARS). The contrast mechanism for image formation depends on vibrational properties of the sample. This method is complementary to a variety of optical microscopies that have emerged recently, which use either linear or non-linear light interactions. Unlike confocal and two-photon fluorescence microscopy, native fluorescence or stain is not required, yet we share the capability of acquiring a stack of images to reconstruct a three dimensional structure.

Keywords

coherent antiStokes Raman scattering; image reconstruction; multiwave mixing; nonlinear optical susceptibility; optical images; optical microscopy; vibrational states; CARS; coherent antiStokes Raman Spectroscopy; coherent antiStokes Raman scattering microscopy; confocal microscopy; contrast mechanism; high resolution microscopy; image formation; images; linear light interactions; native fluorescence; nonlinear light interactions; nonlinear optical method; optical microscopies; reconstruction; stain; three dimensional structure; two-photon fluorescence microscopy; vibrational properties; Frequency; Glass; Laser excitation; Laser tuning; Nonlinear optics; Optical microscopy; Optical mixing; Optical pumping; Raman scattering; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

Quantum Electronics and Laser Science Conference, 1999. QELS '99. Technical Digest. Summaries of Papers Presented at the

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-576-X

Type

conf

DOI

10.1109/QELS.1999.807132

Filename

807132