DocumentCode
3345684
Title
Notice of Retraction
Alternol Inhibits Proliferation and Induces Apoptosis in Human Leukemia HL-60 Cells
Author
Ji Chen ; Defang Li ; Bo Zhang ; Zhenhua Wang ; Yishan Wang ; Qiusheng Zheng
Author_Institution
Key Lab. of Xinjiang Endemic Phytomedicine Resources, Shihezi Univ., Shihezi, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Alternol is purified from fermentation production of microorganisms named as Alternaria alternata var. monosporus. This research is to investigate the apoptosis-inducing by alternol in human pro myelocytic leukemia HL-60 cells. SRB method was used to evaluate the viability of HL-60 cells. Flow cytometry assay was used to examine the apoptosis rate of HL-60 cells and AO/EB staining was used to observe cell morphology. JC-1 staining was used to detect the change of mitochondria membrane potential in HL-60 cells. The proliferation of HL-60 cells was inhibited remarkably in a time-dependent and dose-dependent manner by alternol; the mitochondria membrane potential of HL-60 cells decreased. The results indicate that alternol is a potential agent for leukemia chemo-therapy.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Alternol is purified from fermentation production of microorganisms named as Alternaria alternata var. monosporus. This research is to investigate the apoptosis-inducing by alternol in human pro myelocytic leukemia HL-60 cells. SRB method was used to evaluate the viability of HL-60 cells. Flow cytometry assay was used to examine the apoptosis rate of HL-60 cells and AO/EB staining was used to observe cell morphology. JC-1 staining was used to detect the change of mitochondria membrane potential in HL-60 cells. The proliferation of HL-60 cells was inhibited remarkably in a time-dependent and dose-dependent manner by alternol; the mitochondria membrane potential of HL-60 cells decreased. The results indicate that alternol is a potential agent for leukemia chemo-therapy.
Keywords
bioelectric potentials; biomembranes; blood; cancer; cellular biophysics; drugs; microorganisms; Alternaria alternata var. monosporus; JC-1 staining; alternol; cell apoptosis; cell morphology; cell proliferation; cell viability; chemotherapy; flow cytometry assay; human leukemia HL-60 cells; microorganisms; mitochondria membrane potential; Biomembranes; Cells (biology); Fluorescence; Humans; Microorganisms; Morphology; Proteins;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5781529
Filename
5781529
Link To Document