DocumentCode
33561
Title
CREPE: Mathematical Model for Crosstalking of Endothelial Cells and Hepatocyte Metabolism
Author
Valvano, Giuseppe ; Orsi, Giorgio ; Guzzardi, Maria Angela ; Vozzi, F. ; Vozzi, Giovanni
Author_Institution
Interdept. Res. Center E. Piaggio, Univ. of Pisa, Pisa, Italy
Volume
61
Issue
1
fYear
2014
fDate
Jan. 2014
Firstpage
224
Lastpage
230
Abstract
The liver shows a close coexistence between endothelial cells and hepatocytes (HepG2). Endothelial cells´ main purpose is to protect (HepG2) from blood vessel shear stress, acting as a barrier, but experimental evidence suggests that they could also play a role in regulating (HepG2) glucose metabolism. A well-known singular effect in hepatocyte-endothelial co-cultures is the reduction of glucose consumption respect to (HepG2) in single culture. (HepG2) were shown to reduce their glucose consumption supporting energy needs of endothelial cells. Monti have studied the effects of endothelin-1 (Et-1) on Glucokinase activity in adult rat (HepG2). They observed a reduction in hepatocytes Glucokinase catalytic rate, which is dependent on Et-1 concentration. We developed crosstalking of endothelial cells and hepatocyte metabolism (CREPE) that is a mathematical model of the endothelin-1 mediated crosstalk between HepG2 and endothelial cells (human umbilical vein endothelial cells) in a traditional static co-culture system. CREPE was validated against experimental data, showing good agreement with them. CREPE can be a starting point to develop predictive tools on complex and highly interconnected environments.
Keywords
biochemistry; blood vessels; catalysis; cellular biophysics; physiological models; sugar; CREPE mathematical model; HepG2; blood vessel shear stress; cell crosstalk; endothelin-1; glucose consumption; glucose metabolism; hepatocyte metabolism; hepatocyte-endothelial co-cultures; hepatocytes glucokinase catalytic rate; human umbilical vein endothelial cells; Biochemistry; Biological system modeling; Equations; Genetics; Mathematical model; Substrates; Sugar; Computational systems biology; cell crosstalk; endothelin-1 (Et-1); glucokinase; mathematical modeling;
fLanguage
English
Journal_Title
Biomedical Engineering, IEEE Transactions on
Publisher
ieee
ISSN
0018-9294
Type
jour
DOI
10.1109/TBME.2013.2272942
Filename
6557437
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