Electrically stimulated NGF secretion by astroglial cells

Author

Koyama, Sumihiro ; Haruyama, Tetsuya ; Kobatake, Eiry ; Aizawa, Masuo

Author_Institution

Dept. of Bioeng., Tokyo Inst. of Technol., Yokohama, Japan

Volume

5

fYear

1996

fDate

31 Oct-3 Nov 1996

Firstpage

2061

Abstract

Mouse astrocytes were cultured on an optically transparent electrode (indium tin oxide/glass electrode) and subjected to an electrical sine wave of stimulation at 10 Hz for 1 h. The electrical stimulation of 0 to +0.3 V (vs. Ag/AgCl) peak to peak sine wave enhanced nerve growth factor (NGF) secretion by 6-fold. The mechanistic investigation indicate that the PKC dependent signaling pathway involved in astrocytes could be activated by the electrical stimulation

Keywords

bioelectric phenomena; biological techniques; cellular biophysics; neurophysiology; 0 to 0.3 V; 1 h; 10 Hz; Ag-AgCl; PKC dependent signaling pathway; electrical sine wave; electrical stimulation; indium tin oxide/glass electrode; mechanistic investigation; mouse astrocytes culture; nerve growth factor; optically transparent electrode; Electric shock; Electrical stimulation; Electrodes; Fluids and secretions; Glass; Indium tin oxide; Mice; Production; Proteins; Surface morphology;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE

Conference_Location

Amsterdam

Print_ISBN

0-7803-3811-1

Type

conf

DOI

10.1109/IEMBS.1996.646432

Filename

646432