Spatially Focused Reagent Injection System for Cell Analysis using 3-D Sheath Flow Scanner

Author

Suzuki, Yasuo ; Kanai, Masaki ; Kawai, Kentaro ; Nishimoto, Takahiro ; Shoji, Shuichi

Author_Institution

Waseda Univ., Tokyo

fYear

2007

fDate

10-14 June 2007

Firstpage

25

Lastpage

28

Abstract

We developed a spatially focused reagent injection system using 3-D sheath flow scanner. The proposed 3-D sheath flow scanner enables to introduce reagent specifically to target cell colonies cultivated in a microchamber. SiO₂ mesh strainer is formed in the microwell to improve spatial resolution of the reagent flow. This enables selective delivery of the reagent to target cell colonies cultivated in the microwell. The specific reagent flow is shifted by eight buffer flows which are controlled by four electro- osmotic flow (EOF) pumps. High performance positioning is realized by PC control with a joystick. Observation of confocal fluorescent image confirmed that the reagent flow is focused to small spot of about 100mumPhi on the mesh strainer where biological cells are cultivated.

Keywords

bioMEMS; biological fluid dynamics; biological techniques; biological tissues; cellular biophysics; osmosis; tissue engineering; 3D sheath flow scanner; SiO₂; SiO₂ mesh strainer; biological cells; buffer flows; cell analysis; cell colonies; confocal fluorescent image; electro- osmotic flow pumps; microchamber; microwell; reagent flow; reagent injection system; spatial resolution; Actuators; Argon; Control systems; Fluorescence; Focusing; Nanobioscience; Radio frequency; Residual stresses; Solid state circuits; Sputtering; 3-D sheath flow; EOF pump; cell analysis; micro mesh; sample injector;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300063

Filename

4300063