In Situ Monitoring Drug Effects on Cancer Cell at "Few Cells" Level

Author

Chen, Y. ; Lim, L.H. ; Zhang, Q.X. ; Chong, S.C. ; Tang, K.C. ; Balasubramanian, N.

Author_Institution

Inst. of Microelectron., Singapore

fYear

2007

fDate

10-14 June 2007

Firstpage

1963

Lastpage

1966

Abstract

This research is to develop a system to allow cancer researchers as well as drug discovery people to study drug effects in situ, real-time and at "few cells" level but large cell numbers. A microelectrode array was designed and fabricated by using silicon micromachining technology. The cancer cell NCI-H460 was attached on the Au microelectrode and the high impedance sensitivity at very "few cells" level has been achieved. The results of the anti-alpha2beta1-integrin effects on NCI-H460 lung cancer cells attachment was first time reported. The results obtained here show a good potential to achieve the single cell attachment detection.

Keywords

arrays; bioMEMS; biochemistry; bioelectric phenomena; biomedical measurement; cancer; cellular biophysics; drugs; gold; lung; microelectrodes; molecular biophysics; proteins; tumours; Au; NCI-H460 lung cancer cells; antialpha2beta1-integrin effects; cancer cell; few cells level; impedance measurement system; impedance sensitivity; in situ drug effects monitoring; microelectrode array design; silicon micromachining technology; single cell attachment detection; Cancer; Drugs; Gold; Impedance; Microelectrodes; Micromachining; Monitoring; Pharmaceutical technology; Real time systems; Silicon; anti-cancer drug effects; cancer cell attachment; impedance response; integrin; single cell detection;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300545

Filename

4300545