Low Concentrated DNA Detection by SGFET

In this work a device based on oligonucleotide hybridization charged detection is presented. This biosensor is a SGFET (suspend gate field effect transistor), it is based on a MOSFET (metal oxide semiconductor field effect transistor) structure whose gate is suspended. The insulating layer (empty gap) is chemically modified by immobilized molecular receptors that enable miniaturized label free DNA detection. This method allows direct, time-resolved and in situ detection of specific nucleic acid binding. By the use of SGFET, the immobilization of DNA sequences brings negatives charges due to their phosphate groups under the gate and induce large shift of transfer characteristic. One of the applications of this device can be clinical testing.