Complete gradient-LC-ESI system on a chip for protein analysis

Author

Xie, J. ; Shih, J. ; Miao, Yinping ; Lee, T.D. ; Tai, Y.C.

Author_Institution

California Inst. of Technol., Pasadena, CA, USA

fYear

2005

fDate

30 Jan.-3 Feb. 2005

Firstpage

778

Lastpage

781

Abstract

This paper presents the first fully integrated gradient-elution liquid chromatography-electrospray ionization (LC-ESI) system on a chip. This chip integrates a pair of high-pressure gradient pumps, a sample injection pump, a passive mixer, a packed separation column, and an ESI nozzle. We also present the successful on-chip separation of protein digests by reverse phase (RP)-LC coupled with online mass spectrometer (MS) analysis.

Keywords

chromatography; electron impact ionisation; mass spectroscopic chemical analysis; microfluidics; micropumps; proteins; system-on-chip; ESI nozzle; gradient-LC-ESI system on chip; high-pressure gradient pump; integrated gradient-elution liquid chromatography-electrospray ionization; on-chip separation; online mass spectrometer analysis; packed separation column; passive mixer; protein analysis; protein digests; reverse phase-LC; sample injection pump; Delay; Ionization; Mass spectroscopy; Methanol; Micromechanical devices; Proteins; Proteomics; Research and development; Solvents; Tail;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on

ISSN

1084-6999

Print_ISBN

0-7803-8732-5

Type

conf

DOI

10.1109/MEMSYS.2005.1454045

Filename

1454045