DocumentCode :
3425075
Title :
A novel reusable nanomechanics-based protein biosensor with electrical manipulation
Author :
Hwang, R.-Z. ; Huang, L.-S. ; Chang, Hung-Shuo ; Wu, C.-W. ; Tien, H.-C. ; Lin, S. ; Lee, A.S.-Y.
Author_Institution :
Inst. of Appl. Mech., Nat. Taiwan Univ., Taipei, Taiwan
fYear :
2005
fDate :
30 Jan.-3 Feb. 2005
Firstpage :
798
Lastpage :
801
Abstract :
A novel reusable biosensor of label-free biomolecular recognition based on the nanomechanics transduction in a micro-fabricated cantilever has been developed. The nanomechanics-based immunoassay biosensor was proven to be repeatedly used with desorption of the pre-bound antigen-antibody by an in-situ alternative electrical field. This new approach of electric manipulation in separating the antigen out of the antibody in a buffer fluid environment was confirmed in nanomechanics-based immunoassay biosensor in which a conventional desorption is long used with high concentrated acid solution in a range of pH 2∼3. The potential benefit of the electrical means over a conventional pre-bound antigen-antibody desorption of strong acid solution is to maintain long-standing protein activity in multiple uses such that the micro biosensor is reliable and reproducible in every detection. The complete sensing history from a monolayer of biolinker, to antibody and antigen is also first demonstrated in nanomechanics-based immunoassay.
Keywords :
biosensors; micromanipulators; nanotechnology; proteins; acid solution; alternative electrical field; antigen-antibody separation; biolinker; buffer fluid; desorption; electric manipulation; electrical manipulation; immunoassay biosensor; label-free biomolecular recognition; microbiosensor; microfabricated cantilever; nanomechanics transduction; protein activity; reusable protein biosensor; Biosensors; Electrodes; Gold; Immune system; Nanoscale devices; Nickel; Proteins; Sea surface; Silicon; Stress;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on
ISSN :
1084-6999
Print_ISBN :
0-7803-8732-5
Type :
conf
DOI :
10.1109/MEMSYS.2005.1454050
Filename :
1454050
Link To Document :
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