Title :
Nerve Growth Factor pretreatment promotes vasculogenesis in rats after myocardial infarction
Author :
Li Ying ; Xu Lihua ; Zhan Ping ; Sun Miying ; Zhai Yujia ; Xu Lei ; Liu Tongku
Author_Institution :
Dept. of Cardiology, Affiliated Hosp. of Beihua Univ., Jilin, China
Abstract :
To investigate the effects of NGF(Nerve Growth Factor) pretreatment on density of new vessels of acute myocardial infaction in rats. 60 male adult Wistar rats were randomly classified into three groups: the control (n=20), non-NGF (n=20) and NGF(n=20) groups. The NGF group was injected NGF (0.5μg/kg) via caudal vena. Then we performed left anterior descending coronary artery(LAD) ligation, all the rats were sacrificed at 7 days postsurgery. In this study we observe the morphologic changes of myocardial pathology and the serum biochemical changes in each group, including CK-MB, LDH and CnTI; To measure the expression of CD34, VEGF and KDR/flk-1 we use immunohistology and RT-PCR. Compared with the control group and the non-NGF group, the NGF group has a significant higher density of vessel(P<;0.01). Our results indicated that NGF pretreatment can enhance the density of new vessels, promote the vasculogenesis and lessen the myocardial damage in rats.
Keywords :
biochemistry; biological tissues; blood vessels; cardiology; cellular biophysics; diseases; molecular biophysics; neurophysiology; proteins; surgery; CD34 expression; KDR-flk-1 expression; VEGF expression; acute myocardial infaction; coronary artery ligation; immunohistology; male adult Wistar rats; morphologic changes; myocardial pathology; nerve growth factor pretreatment; postsurgery; serum biochemical changes; vasculogenesis; Arteries; Heart; Injuries; Myocardium; Rats; Software; Myocardial infarction; Nerve Growth Factor; Vasculogenesis; rat;
Conference_Titel :
Human Health and Biomedical Engineering (HHBE), 2011 International Conference on
Conference_Location :
Jilin
Print_ISBN :
978-1-61284-723-8
DOI :
10.1109/HHBE.2011.6028039
