The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging

Author

Holtom, G.R. ; Thrall, B.D. ; Chin, B.Y. ; Risby, T.H.

Author_Institution

Environ. Molecular Sci. Lab., Pacific Northwest Lab., Richland, WA, USA

fYear

2001

fDate

11-11 May 2001

Firstpage

362

Lastpage

363

Abstract

Summary form only given. Coherent Anti-Stokes Raman Spectroscopy (CARS) has been applied to microscopic imaging and is similar in method and application to two-photon fluorescence microscopy. A Stokes-shifted laser source (an optical parametric amplifier operating at 250 kHz), in combination with the 200 fs pump laser, is tightly focused into the sample, with high peak power but with nanojoule pulse energy. The stimulated signal is orders of magnitude stronger than conventional Raman signals and in addition has a very high contrast due to insignificant autofluorescence.

Keywords

Raman spectroscopy; biological techniques; fluorescence; molecular biophysics; nonlinear optics; optical microscopy; optical parametric amplifiers; 200 fs; 250 kHz; 3-D biological imaging; autofluorescence; biophysical research technique; coherent antiStokes Raman spectroscopy; molecular specificity; nanojoule pulse energy; nonlinear Raman spectroscopy; pump laser; stimulated signal; two-photon fluorescence microscopy; Fluorescence; Microscopy; Nonlinear optics; Optical imaging; Power lasers; Pulse amplifiers; Pump lasers; Raman scattering; Spectroscopy; Stimulated emission;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2001. CLEO '01. Technical Digest. Summaries of papers presented at the Conference on

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-662-1

Type

conf

DOI

10.1109/CLEO.2001.947916

Filename

947916