Title :
Phaseguide-chip for point-of-care diagnostic of bacteria with integrated enrichment, lysis, and nucleic acid purification
Author :
Hubbe, H. ; Hakenberg, S. ; Dame, G. ; Urban, G.A.
Author_Institution :
Lab. for Sensors, IMTEK Univ. of Freiburg, Freiburg, Germany
Abstract :
We report the development and proof-of-concept of a microfluidic chip, that provides a full range of pretreatment steps for the point-of-care detection of bacteria with a total process time of less than 15 minutes. It integrates enrichment, lysis and nucleic acid purification. Enrichment is achieved through free-flow electrophoresis. The enriched cells are lysed by thermoelectrical means. Nucleic acids can immediately be purified by electrophoresis through a hydrogel. The system uses phaseguides for complete, bubble-free priming and emptying, offering optional reusability.
Keywords :
bioMEMS; biochemistry; bioelectric phenomena; biomedical equipment; cellular biophysics; electrophoresis; hydrogels; macromolecules; microfluidics; microorganisms; molecular biophysics; patient diagnosis; thermoelectricity; bubble-free emptying; bubble-free priming; electrophoresis; hydrogel; integrated enrichment; lysis; microfluidic chip; nucleic acid purification; offering optional reusability; phase guide-chip; point-of-care bacteria detection; point-of-care bacteria diagnosis; thermoelectrical means; Electrodes; Microfluidics; Microorganisms; Pathogens; Purification; Sensors; Substrates; Lab-on-a-chip; pathogen detection; point-of-care diagnostic; sample pretreatment;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
DOI :
10.1109/Transducers.2013.6627223