Motor protein based tau protein detection device

Author

Orazov, Y. ; Tarhan, M.C. ; Yokokawa, Ryuji ; Karsten, S.L. ; Fujita, Hideaki

Author_Institution

Univ. of Tokyo, Tokyo, Japan

fYear

2013

fDate

16-20 June 2013

Firstpage

2447

Lastpage

2450

Abstract

A microfluidics device to detect microtubule-binding proteins was proposed using microtubule-kinesin based system. To provide “proof of principle”, attachment of microtubule associated tau protein was detected via the change in kinesin velocity. The proposed device is capable of multiplex parallel assay of several experimental samples by using the same kinesin-coated beads moving in the same channel with immobilized microtubules. The results showed that the device successfully detected the hindered kinesin motion due to tau attachment on microtubule (MT) surface.

Keywords

bioMEMS; biomedical equipment; microfluidics; molecular biophysics; proteins; hindered kinesin motion; immobilized microtubules; kinesin velocity; kinesin-coated beads; microfluidics device; microtubule surface; microtubule-binding proteins; microtubule-kinesin-based system; motor protein; tau protein detection device; Alzheimer´s disease; Fluids; Microfluidics; Monitoring; Performance evaluation; Proteins; Molecular detection; kinesin; microtubules; tau protein;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6627301

Filename

6627301