Title :
Motor protein based tau protein detection device
Author :
Orazov, Y. ; Tarhan, M.C. ; Yokokawa, Ryuji ; Karsten, S.L. ; Fujita, Hideaki
Author_Institution :
Univ. of Tokyo, Tokyo, Japan
Abstract :
A microfluidics device to detect microtubule-binding proteins was proposed using microtubule-kinesin based system. To provide “proof of principle”, attachment of microtubule associated tau protein was detected via the change in kinesin velocity. The proposed device is capable of multiplex parallel assay of several experimental samples by using the same kinesin-coated beads moving in the same channel with immobilized microtubules. The results showed that the device successfully detected the hindered kinesin motion due to tau attachment on microtubule (MT) surface.
Keywords :
bioMEMS; biomedical equipment; microfluidics; molecular biophysics; proteins; hindered kinesin motion; immobilized microtubules; kinesin velocity; kinesin-coated beads; microfluidics device; microtubule surface; microtubule-binding proteins; microtubule-kinesin-based system; motor protein; tau protein detection device; Alzheimer´s disease; Fluids; Microfluidics; Monitoring; Performance evaluation; Proteins; Molecular detection; kinesin; microtubules; tau protein;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
DOI :
10.1109/Transducers.2013.6627301
