Title :
Nanosecond pulsed electric fields inhibit breast cancer development and suppress tumor blood vessel growth
Author :
Wu, Shiqian ; Wang, Yannan ; GUO, Jun ; Chen, Qian ; Zhang, Juyong ; Fang, Jianwu
Author_Institution :
Peking Univ., Beijing, China
Abstract :
Nanosecond pulsed electric fields (nsPEFs) is a novel non-thermal approach to induce cell apoptosis. In this study, we established breast cancer animal model with MCF-7 cell line on Balb/c nude mice. 30 tumors were exposed to 720 pulses of 100ns at 30kV/cm. Treated tumors was inhibited by 79% 2 weeks after pulse. A 3.0T clinical magnetic resonance imaging (MRI) system with a self-made surface coil was employed to observe morphological changes of tumors. Pulsed tumors showed histological markers of apoptosis and decreased blood vessel density. To evaluate the might-be adverse effects of nsPEFs in healthy tissues, normal skin was treated in exactly the same way as tumors, and pulsed skin showed no permanent damages. The results suggest nsPEFs is able to inhibit human breast cancer development and suppress tumor blood vessel growth, indicating nsPEFs may serve as a novel therapy for breast cancer in the future.
Keywords :
biological organs; biomedical MRI; blood vessels; cancer; cellular biophysics; coils; patient treatment; skin; tumours; MCF-7 cell line; MRI; blood vessel density; breast cancer animal model; breast cancer therapy; cell apoptosis; clinical magnetic resonance imaging; healthy tissues; human breast cancer development; magnetic flux density 3.0 T; nanosecond pulsed electric fields; nonthermal approach; nude mice; pulsed tumors; skin; surface coil; tumor blood vessel growth; Biomedical imaging; Blood vessels; Breast cancer; Electric fields; Mice; Skin; Tumors;
Conference_Titel :
Pulsed Power Conference (PPC), 2013 19th IEEE
Conference_Location :
San Francisco, CA
DOI :
10.1109/PPC.2013.6627554
