DocumentCode :
3494602
Title :
Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells
Author :
Hsiao, A.Y. ; Okitsu, Takashi ; Onoe, H. ; Kiyosawa, M. ; Teramae, H. ; Iwanaga, Shinya ; Miura, Shun ; Kazama, Takushi ; Matsumoto, Tad ; Takeuchi, Shoji
Author_Institution :
Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan
fYear :
2013
fDate :
20-24 Jan. 2013
Firstpage :
71
Lastpage :
73
Abstract :
This paper describes the self-assembly of “cell springs” using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.
Keywords :
bioMEMS; bioelectric phenomena; cellular biophysics; microfabrication; microfluidics; mixtures; molecular biophysics; muscle; proteins; self-assembly; 3D fiber-shaped microconstruction; DFAT cell fibers; cell springs; coiled spring structures; collagen; differentiation induction; electrical stimulation; extracellular matrix mixture; fibrin; microfluidic coaxial device; multipotent dedifferentiated fat cells; self-assembly; smooth muscle-like cell differentiation; synchronized contraction; tubular alginate microfibers; Electronic countermeasures; Media; Muscles; Optical fiber amplifiers; Optical fiber devices; Self-assembly; Springs;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Conference_Location :
Taipei
ISSN :
1084-6999
Print_ISBN :
978-1-4673-5654-1
Type :
conf
DOI :
10.1109/MEMSYS.2013.6474179
Filename :
6474179
Link To Document :
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