Title :
Microfluidic device for the continuous preparation of eukaryotic cells for metabolic analysis
Author :
Rajabi, N. ; Bahnemann, J. ; Tzeng, T. ; Zeng, A. ; Muller, Johannes
Author_Institution :
Inst. of Microsyst. Technol., Hamburg Univ. of Technol., Hamburg, Germany
Abstract :
A novel lab-on-a-chip is presented for the high-throughput preparation of mammalian cell samples. The microfluidic chip consists of two temperature zones and five functional modules with a total number of four fluidic inlet and three outlet channels for sample collection. The key modules of the chip are a rapid split-and-recombine micromixer for biological system perturbation, a chaotic advection “stirred” incubation channel for lowered residence time distribution, and a spiral channel for efficient particle separation and media exchange. The passive chip is suitable for dynamic pulse experiments for metabolic analyses in cell compartments.
Keywords :
bioMEMS; biological specimen preparation; cellular biophysics; chaos; lab-on-a-chip; microchannel flow; mixing; perturbation theory; biological system perturbation; cell compartments; chaotic advection stirred incubation channel; continuous eukaryotic cell preparation; dynamic pulse experiments; fluidic inlet; functional modules; high-throughput mammalian cell sample preparation; lab-on-a-chip; lowered residence time distribution; metambolic analysis; microfluidic chip; microfluidic device; outlet channels; particle separation; rapid split-and-recombine micromixer; sample collection; spiral channel; Fluorescence; Lab-on-a-chip; Microfluidics; Mixers; Spirals; Sugar;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Conference_Location :
Taipei
Print_ISBN :
978-1-4673-5654-1
DOI :
10.1109/MEMSYS.2013.6474227
