DocumentCode :
3534894
Title :
Antioxidant potential of phytonanoparticles in an emerging biofluid (saliva) of periodontitis patients
Author :
Amarnath, K. ; Nellore, Jayshree
Author_Institution :
Dept. of Biochem., Sathyabama Univ., Chennai, India
fYear :
2011
fDate :
28-30 Nov. 2011
Firstpage :
401
Lastpage :
407
Abstract :
Periodontal disease is associated with an imbalance between oxidants and antioxidants, due to both an increase in free radical production and a defect in the total antioxidant activity of the oral fluid, saliva. Nano-sized drug carriers with highly controlled shapes, sizes play an important role in antioxidant activity thereby improving the bioavailability of drug with poor absorption characteristics. Henceforth we aimed to synthesize a novel nano antioxidant by ionic gelation method using carboxymethyl chitosan and chitosan hydrochloride as carriers of grape seed polyphenols (CS-GSPNps). The characteristics of CS-GSPNps were determined by using TEM and FTIR spectroscopy and found that the synthesized CS- GSPNps were non-spherical in shape with an average size of 400nm. Further we determined the antioxidant effect of CS- GSPNps and grape seed extract (GSE) on the salivary antioxidant potential, LPO levels and salivary biomarker enzymes in patients with periodontitis (10) versus controls (10). Statistical analysis revealed changes in the above mentioned parameters which was found to be significant (P>;0.001) upon CS- GSPNps treatment rather than treated with GSE. Based on these results, CS-GSPNps prepared in our present system are promising candidates for therapy effects in periodontal disease.
Keywords :
biomedical materials; botany; dentistry; diseases; drugs; enzymes; nanomedicine; nanoparticles; CS-GSPNps; FTIR spectroscopy; TEM; antioxidant activity; bioavailability; biofluid; carboxymethyl chitosan; chitosan hydrochloride; grape seed polyphenols; ionic gelation; nanosized drug carriers; periodontitis; phytonanoparticles; saliva; statistical analysis; Europe; Liver; Nanoparticles; Neuroscience; Polymers; Antioxidant enzymes; CS-GSPNps; Saliva; oxidative stress; periodontal disease; salivary biomarkers; total antioxidant capacity;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nanoscience, Engineering and Technology (ICONSET), 2011 International Conference on
Conference_Location :
Chennai
Print_ISBN :
978-1-4673-0071-1
Type :
conf
DOI :
10.1109/ICONSET.2011.6167989
Filename :
6167989
Link To Document :
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