Diode-pumped lasers for two-photon fluorescence microscopy

Author

Robertson, G. ; Armstrong, David ; Dymott, M.J.P. ; Ferguson, A.I. ; Hogg, Graeme

Author_Institution

Dept. of Phys. & Appl. Phys., Strathclyde Univ., Glasgow, UK

fYear

1996

fDate

2-7 June 1996

Firstpage

103

Abstract

Summary form only given. We report the use of diode-pumped mode-locked lasers for generating two-photon fluorescence microscopy (TPFM) images. TPFM offers several advantages over the more conventional confocal fluorescence microscopy, namely improved depth penetration through samples, confinement of dye bleaching to the focus area, and a reduction in the background fluorescence levels caused by out-of-focus fluorescence. In addition, the absorption confinement reduces phototoxicity for live samples.

Keywords

fluorescence; laser beam applications; optical microscopy; optical pumping; solid lasers; two-photon processes; absorption confinement; background fluorescence; depth penetration; diode-pumped mode-locked laser; dye bleaching; live sample; phototoxicity; two-photon fluorescence microscopy; Diodes; Fluorescence; Laser mode locking; Laser theory; Microcavities; Microscopy; Power system reliability; Semiconductor lasers; Surface emitting lasers; Vertical cavity surface emitting lasers;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 1996. CLEO '96., Summaries of papers presented at the Conference on

Conference_Location

Anaheim, CA, USA

Print_ISBN

1-55752-443-2

Type

conf

Filename

864421