Title :
Rapid determination of cellular metabolic rates with a silicon microphysiometer
Author :
Bousse, L.J. ; Miller, D.L. ; Owicki, J.C. ; Parce, J.W.
Author_Institution :
Molecular Devices Corp., Menlo Park, CA, USA
Abstract :
The microphysiometer is a silicon-sensor based instrument designed to measure the metabolic rates of small numbers of living cells. By improving the electronic noise level of this instrument, the authors have increased the temporal resolution of metabolic rate measurement by about an order of magnitude compared to previous versions of the instrument. This enables the study of relatively fast cellular responses to receptor/ligand binding. Results obtained with a cell line (TE671) that is known to contain acetylcholine receptors are presented. The kinetics of the cellular response to an acetylcholine agonist is shown to be complex, with three distinct resolvable phases. Through pharmacological manipulations it is demonstrated that the response is due to a muscarinic acetylcholine receptor.<>
Keywords :
biological techniques and instruments; biomedical measurement; biosensors; cellular biophysics; electric sensing devices; elemental semiconductors; pH measurement; silicon; Si sensor; acetylcholine agonist; acetylcholine receptors; biosensor; cellular metabolic rates; cellular responses; electronic noise; kinetics; light addressable potentiometric sensor; living cells; metabolic rate measurement; microphysiometer; muscarinic acetylcholine receptor; pharmacological manipulations; receptor/ligand binding; temporal resolution; Biochemistry; Biosensors; Drugs; In vitro; Instruments; Noise measurement; Optoelectronic and photonic sensors; Silicon; Sugar; Toxicology;
Conference_Titel :
Solid-State Sensors and Actuators, 1991. Digest of Technical Papers, TRANSDUCERS '91., 1991 International Conference on
Conference_Location :
San Francisco, CA, USA
Print_ISBN :
0-87942-585-7
DOI :
10.1109/SENSOR.1991.148802
