An AC electroosmotic processor for biomolecules

Author

Pak Kin Wong ; Che-Yang Chen ; Tza-Huei Wang ; Chih-Ming Ho

Author_Institution

Dept. of Mech. & Aerosp. Eng., California Univ., Los Angeles, CA, USA

Volume

1

fYear

2003

Firstpage

20

Abstract

This paper describes an AC electrokinetic processor for biomedical analysis systems. The processor exploits AC electroosmosis for concentrating bioparticles, such as cells and molecules. Particle image velocimetry (PIV) is used to characterize the AC electroosmotic flow. A combination of the AC electroosmotic flow and other electrokinetic forces allows collection and concentration of the bioparticles. By optimizing the operating parameters, we have successfully demonstrated concentration of various biological objects, such as E. coli bacteria, /spl lambda/ phage DNA, and single-strand DNA fragments as small as 20 bases.

Keywords

DNA; biomedical materials; electrokinetic effects; electrophoresis; microfluidics; microorganisms; molecular biophysics; osmosis; /spl lambda/ phage DNA; AC electrokinetic forces; AC electroosmotic processor; E. coli bacteria; biomolecules; cells; concentrating bioparticles; electroosmosis; molecules; particle image velocimetry; single-strand DNA fragments; Aerospace engineering; Biochemical analysis; Biomedical engineering; DNA; Electrodes; Electrokinetics; Fabrication; Microfluidics; Microorganisms; Molecular biophysics;

fLanguage

English

Publisher

ieee

Conference_Titel

TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003

Print_ISBN

0-7803-7731-1

Type

conf

DOI

10.1109/SENSOR.2003.1215243

Filename

1215243