A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability

Author

Wenpeng Xun ; Jianguo Feng ; Honglong Chang

Author_Institution

Minist. of Educ. Key Lab. of Micro & Nano Syst. for Aerosp., Northwestern Polytech. Univ., Xi´an, China

Volume

14

Issue

8

fYear

2015

Firstpage

850

Lastpage

856

Abstract

In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.

Keywords

bioMEMS; blood; cellular biophysics; fluorescence; health care; lab-on-a-chip; microfluidics; PDMS microfluidic chip; coefficient-of-variation; disposable microfluidic chip; double molding process; flow-check alignment beads; fluorescein isothiocyanate channel; fluorescence detection capability; lymphocyte gating; point-of-care microflow cytometers; polydimethylsiloxane; sheathless focus mechanism; side scatter detection; Detectors; Fluorescence; Focusing; Histograms; Laser beams; Measurement by laser beam; Microfluidics; Flow cytometer; fluorescence; microfluidics; polydimethylsiloxane; side scatter;

fLanguage

English

Journal_Title

NanoBioscience, IEEE Transactions on

Publisher

ieee

ISSN

1536-1241

Type

jour

DOI

10.1109/TNB.2015.2455073

Filename

7277090