DocumentCode :
3734935
Title :
A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood
Author :
I-Fang Cheng;Tzu-Ying Chen;Yu-De Lin;Wei-Lung Huang;Chien-Wei Liu;Wu-Chou Su
Author_Institution :
National Nano Device Laboratories, National Applied Research Laboratories, Tainan, Taiwan
fYear :
2015
fDate :
7/1/2015 12:00:00 AM
Firstpage :
1525
Lastpage :
1527
Abstract :
An antibody-free approach for high purity and high throughput isolation of circulating tumor cells (CTCs) from human blood in a microfluidic chip is presented. High throughput continuous CTC isolation (>1.2 ml/hr) is achieved with a sustained dielectrophoresis (DEP) particle force normal to the continuous through-flow. The design allows continuous fractionation of cells into different downstream sub-channels based on the differences in their different critical DEP strengths/mobility on both sides of the cross-over. An enrichment factor of ~105 and a recovery rate of ~80% from a 0.001% CTC sample is achieved at a high throughput of 1.2 ml/hr. Compared to conventional physical methods, such as immunomagnetics-based methods and microfluidic biomarker-based methods, the isolation purity, recovery rate, throughput and viability of the proposed antibody-free chip demonstrated excellent capabilities, especially with regard to high enrichment at a high flow rate.
Keywords :
"Cells (biology)","Blood","Tumors","Electric fields","Microfluidics","Cancer","Laboratories"
Publisher :
ieee
Conference_Titel :
Nanotechnology (IEEE-NANO) , 2015 IEEE 15th International Conference on
Type :
conf
DOI :
10.1109/NANO.2015.7388934
Filename :
7388934
Link To Document :
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