Title :
Micropatterned three-dimensional culture in click-crosslinkable and photodegradable gelatin hydrogels
Author :
Masato Tamura;Fumiki Yanagawa;Shinji Sugiura;Toshiyuki Takagi;Kimio Sumaru;Toshiyuki Kanamori
Author_Institution :
Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan
Abstract :
This paper reports a novel click-crosslinkable and photodegradable gelatin hydrogels, which is useful for micropatterned three-dimensional culture. The azide-modified gelatin was prepared by mixing gelatin with Azide-PEG4-NHS ester solutions. The DBCO-PC-4armPEG was synthesized by the reaction between NHS-PC-4armPEG and DBCO-PEG4-amine. PD-gelatin was performed by mixing solutions of azide-modified gelatin and DBCO-PC-4armPEG in a 1:1 ratio. We successfully demonstrated the micropatterning of the photodegradable gelatin hydrogels and cell encapsulation in these hydrogels.
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2015 International Symposium on
DOI :
10.1109/MHS.2015.7438240
