Title :
Effects of surface chemistry and protein competitive adsorption on cell adhesion
Author :
Ying, P.-Q. ; Jin, G. ; Tao, Z.L.
Author_Institution :
Inst. of Mech., Acad. Sinica, Beijing, China
Abstract :
The competitive adsorption between collagen and bovine serum albumin (BSA) was investigated by imaging ellipsometry. The competitive adsorption of the binary solution depended on the surface chemistry of substrates. At the range of concentration investigated, collagen preferentially adsorbed on the hydroxy surface (-OH surface) but BSA on the methylic surface (-CH3 surface). ROS 17/2.8 cell adhesion on the two kinds of surfaces with or without the pre-adsorption of protein was also investigated. The results showed that the competitive adsorption caused ROS 17/2.8 cells to preferentially adhere and spread well on the -OH surface, but they had less adherence and didn´t spread on the -CH3 surface.
Keywords :
adhesion; adsorption; biochemistry; cellular biophysics; ellipsometry; proteins; substrates; surface chemistry; CH3 surface; OH surface; ROS cell adhesion; adherence; binary solution; bovine serum albumin; cell adhesion; collagen; competitive adsorption; concentration; hydroxy surface; imaging ellipsometry; methylic surface; pre-adsorption; protein competitive adsorption; spreading; substrates; surface chemistry; Adhesives; Chemistry; Electrochemical machining; Ellipsometry; Optical imaging; Pollution measurement; Protein engineering; Silicon compounds; Surface contamination; Visualization;
Conference_Titel :
Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE
Print_ISBN :
0-7803-7211-5
DOI :
10.1109/IEMBS.2001.1017412
