Effects of surface chemistry and protein competitive adsorption on cell adhesion

Author

Ying, P.-Q. ; Jin, G. ; Tao, Z.L.

Author_Institution

Inst. of Mech., Acad. Sinica, Beijing, China

Volume

3

fYear

2001

fDate

2001

Firstpage

2957

Abstract

The competitive adsorption between collagen and bovine serum albumin (BSA) was investigated by imaging ellipsometry. The competitive adsorption of the binary solution depended on the surface chemistry of substrates. At the range of concentration investigated, collagen preferentially adsorbed on the hydroxy surface (-OH surface) but BSA on the methylic surface (-CH₃ surface). ROS 17/2.8 cell adhesion on the two kinds of surfaces with or without the pre-adsorption of protein was also investigated. The results showed that the competitive adsorption caused ROS 17/2.8 cells to preferentially adhere and spread well on the -OH surface, but they had less adherence and didn´t spread on the -CH₃ surface.

Keywords

adhesion; adsorption; biochemistry; cellular biophysics; ellipsometry; proteins; substrates; surface chemistry; CH₃ surface; OH surface; ROS cell adhesion; adherence; binary solution; bovine serum albumin; cell adhesion; collagen; competitive adsorption; concentration; hydroxy surface; imaging ellipsometry; methylic surface; pre-adsorption; protein competitive adsorption; spreading; substrates; surface chemistry; Adhesives; Chemistry; Electrochemical machining; Ellipsometry; Optical imaging; Pollution measurement; Protein engineering; Silicon compounds; Surface contamination; Visualization;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7211-5

Type

conf

DOI

10.1109/IEMBS.2001.1017412

Filename

1017412