Cell clinics for bioelectronic interface with single cells

Author

Abshire, P. ; Lauenstein, J.-M. ; Liu, Y. ; Smela, E.

Author_Institution

Electr. & Comput. Eng., Maryland Univ., College Park, MD, USA

Volume

3

fYear

2003

fDate

25-28 May 2003

Abstract

We describe integrated microstructures and instrumentation for capture and characterization of large numbers of individual cells. Each of these "cell clinics" consists of a cell-sized cavity and a lid that can be opened and closed by hinges constructed from polypyrrole microactuators linking rigid plates to the substrate. These clinics are fabricated on integrated circuit substrates to bring sensors, actuation, computation, and control together at the scale of a single cell. Sensing modalities are tailored for specific applications; we have designed and begun to fabricate and test prototypes of cell clinics for impedance measurements, extracellular recording and stimulation of electrically active cells, and optical measurements.

Keywords

CMOS integrated circuits; application specific integrated circuits; bioelectric phenomena; biological techniques; biosensors; cellular biophysics; microactuators; microelectrodes; microsensors; amplifiers; bioelectronic interface; cell clinics; cell-sized cavity; custom integrated CMOS electronics; dissociated adult Xenopus laevis brainstem neurons; electrically active cell stimulation; extracellular recording; filters; hinges; impedance measurements; integrated circuit substrates; integrated microstructures; lid; low-temperature post-processing steps; optical detection circuitry; optical measurements; polypyrrole microactuators; sensing modalities; sensors; silicon substrate; single cell biosensors; single cells; Circuit testing; Fasteners; Impedance measurement; Instruments; Joining processes; Microactuators; Microstructure; Optical design; Optical recording; Sensor phenomena and characterization;

fLanguage

English

Publisher

ieee

Conference_Titel

Circuits and Systems, 2003. ISCAS '03. Proceedings of the 2003 International Symposium on

Print_ISBN

0-7803-7761-3

Type

conf

DOI

10.1109/ISCAS.2003.1205095

Filename

1205095