Title :
All optical histology of brain tissue: serial ablation and multiphoton imaging with femtosecond laser pulses
Author :
Tsai, Philbert S. ; Friedman, Beth ; Lev-Ram, Varda ; Xiong, Qing ; Tsien, Roger Y. ; Kleinfeld, David ; Ifarraguerri, Agustin I. ; Thompson, Beverly D. ; Squier, Jeff A.
Author_Institution :
Dept. of Phys. & Pharmacology, California Univ., San Diego, La Jolla, CA, USA
Abstract :
We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
Keywords :
biological effects of laser radiation; biomedical optical imaging; blood vessels; brain; cellular biophysics; high-speed optical techniques; iterative methods; laser ablation; multiphoton processes; brain tissue; diffraction-limited volumetric data; femtosecond laser pulse; labeled cells; microvasculature; multiphoton imaging; optical histology; serial ablation; successive iteration; Brain; Fluorescence; Laser ablation; Optical imaging; Optical pulses; Optical scattering; Proteins; Rough surfaces; Surface roughness; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 2003. CLEO '03. Conference on
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-748-2
