Picosecond conformational transition of a cyclic peptide observed by infrared spectroscopy

Author

Sieg, A. ; Schrada, T. ; Zinth, Wofgang ; Wachtveitl, J. ; Renner, Christian ; Behrendt, Raymond ; Moroder, Luis ; Bredenbeck, Jens ; Helbing, Jan ; Hamm, Peter

Author_Institution

Phys. Chem. Inst., Zurich Univ., Switzerland

fYear

2003

fDate

22-27 June 2003

Firstpage

171

Abstract

By visible-pump/infrared-probe spectroscopy, a cyclic octapeptide with a photoswitchable azobenzene based pseudo amino acid (AMPB) embedded in its backbone is investigated. The linear corresponding molecule is used as a reference. The AMPB molecule can be switched by light between a cis and trans conformation. NMR investigations on bcAMPB show an ensemble of a few structures for the cis state and a well defined one for the trans state. A visible pump pulse is used to start the conformational change by switching the AMPB from cis to trans. The probe pulse covers a range of 1600 cm^-1 to 1750 cm^-1 with a time resolution of 0.7 ps.

Keywords

bio-optics; biological NMR; high-speed optical techniques; infrared spectra; molecular biophysics; molecular configurations; proteins; 0.7 ps; NMR; cis state; cis transformation; cyclic octapeptide; cyclic peptide; infrared spectroscopy; photoswitchable azobenzene; picosecond conformational transition; pseudoamino acid; trans conformation; trans state; visible pump pulse; visible-pump/infrared-probe spectroscopy; Absorption; Computational Intelligence Society; Cooling; Delay; Frequency; Infrared spectra; Peptides; Probes; Proteins; Winches;

fLanguage

English

Publisher

ieee

Conference_Titel

Quantum Electronics Conference, 2003. EQEC '03. European

Print_ISBN

0-7803-7733-8

Type

conf

DOI

10.1109/EQEC.2003.1314028

Filename

1314028