Real-time estimation of oxygen concentration in micro-hemo-vessels

Author

De Francisci, M. ; Bucolo, M. ; Intaglietta, M. ; Arena, P. ; Fortuna, L.

Author_Institution

Dipt. di Ingegneria Elettrica, Elettronica e dei Sistemi, Univ. degli Studi di Catania, Italy

Volume

1

fYear

2004

fDate

1-5 Sept. 2004

Firstpage

2231

Lastpage

2234

Abstract

A real-time measurement system for non-invasive evaluation of oxygen concentration (PO₂) at the microcirculation level is developed. The system has been designed by exploiting the phenomenon of fluorescence quenching. The skin of an anaesthetized hamster, injected with porphyrin, is lighted with pulses; the fluorophore reacts with the oxygen in the blood, producing a fluorescence signal, and the value of the fluorescence lifetime is related to the oxygen concentration. This microcirculation-based instrumentation consists of an electro-optical system, a control circuit and signal processing procedure. The system allows the measurement of PO₂ in the range of 0-700 (mmHg) with a standard deviation of 4 (mmHg). Several experiments have been performed in order to characterize and test this system.

Keywords

biomedical equipment; blood vessels; electro-optical devices; fluorescence; haemorheology; medical signal processing; oxygen; radiation quenching; skin; anaesthetized hamster; blood; control circuit; electro-optical system; fluorescence lifetime; fluorescence quenching; fluorophore; micro-hemo-vessels; microcirculation; porphyrin; real-time oxygen concentration estimation; signal processing; skin; Blood; Circuits; Control systems; Fluorescence; Instruments; Measurement standards; Performance evaluation; Real time systems; Signal processing; Skin; Microciculation; measurament system; oxygen concentration; quenching of the fluorescence;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE

Conference_Location

San Francisco, CA

Print_ISBN

0-7803-8439-3

Type

conf

DOI

10.1109/IEMBS.2004.1403650

Filename

1403650