Imaging Cellular Calcium Dysfunction in the Heart Using Multi-Modal Optical Mapping

Author

Hoeker, Gregory S. ; Katra, Rodolphe P. ; Laurita, Kenneth R.

Author_Institution

Dept. of Biomed. Eng., Case Western Reserve Univ., Cleveland, OH

fYear

2006

fDate

Aug. 30 2006-Sept. 3 2006

Firstpage

571

Lastpage

575

Abstract

To study calcium mediated arrhythmogenesis, we have developed a multi-modal optical mapping system that is able to perform dual calcium voltage and ratiometric calcium optical mapping in the same cardiac preparation. To ensure physiological stability of the preparation, the ECG, coronary pressure, coronary flow, and perfusion temperature are continuously monitored throughout each experiment. The multi-modal optical mapping system has been designed so that it can be easily switched from ratiometric calcium mode to dual calcium-voltage mode. It is concluded that multi-modal optical mapping is a very powerful technique for investigating calcium mediated arrhythmogenesis

Keywords

biochemistry; biomedical optical imaging; biothermics; calcium; cellular biophysics; diseases; electrocardiography; haemorheology; ECG; calcium mediated arrhythmogenesis; cardiac preparation; cellular calcium dysfunction imaging; coronary flow; coronary pressure; dual calcium voltage; heart dysfunction; multimodal optical mapping; perfusion temperature; physiological stability; ratiometric calcium optical mapping; Biomedical monitoring; Calcium; Electrocardiography; Heart; Optical design; Optical imaging; Stability; Temperature measurement; Temperature sensors; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE

Conference_Location

New York, NY

ISSN

1557-170X

Print_ISBN

1-4244-0032-5

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2006.259732

Filename

4461814