Surface Functionalization for Protein Microarray

Author

Simion, Monica ; Ruta, Lavinia ; Kleps, Irina ; Mihailescu, Carmen ; Ignat, Teodora ; Stan, Dana ; Craciunoiu, Florin ; Miu, Mihaela ; Bragaru, Adina

Author_Institution

Nat. Inst. for Reserch & Dev. in Microtechnologies (IMT), Bucharest

Volume

1

fYear

2007

fDate

Oct. 15 2007-Sept. 17 2007

Firstpage

135

Lastpage

138

Abstract

This paper presents an attempt to use two substrate types silicon and glass for microarray technique, in order to find the best chemistry surface for immobilization of proteins in microarray technology. Different chemical substrate were studied (glass slides and silicon slides coated with gold and poly-L-lysine) printed with BSA and marked with CY3. The slides were printed and after that washed with PBS (pH=7.4) 20, 40 and 60 minutes. The intensity of the fluorescent signal of the spots before and after washing was analyzed using Gene TAC Analyser (Genomic Solutions) and the UV spectra of the washing residues were recorded.

Keywords

bio-optics; fluorescence; molecular biophysics; proteins; surface chemistry; ultraviolet spectra; Gene TAC Analyser; Si-Au; SiO₂-Au; UV spectra; bovine serum albumin; fluorescent signal intensity; glass substrate; gold; pH; poly-L-lysine; protein microarray; silicon substrate; surface chemistry; surface functionalization; time 20 min; time 40 min; time 60 min; washing residues; Bioinformatics; Chemical technology; Chemistry; Fluorescence; Genomics; Glass; Gold; Proteins; Signal analysis; Silicon; protein microarray; surface functionalisation;

fLanguage

English

Publisher

ieee

Conference_Titel

Semiconductor Conference, 2007. CAS 2007. International

Conference_Location

Sinaia

ISSN

1545-827X

Print_ISBN

978-1-4244-0847-4

Type

conf

DOI

10.1109/SMICND.2007.4519665

Filename

4519665