Miniaturized real-time PCR system: Toward smart diagnostic device for point-of-care food pathogens DNA analyze

Human illnesses caused by food pathogen are cost-generating problem in societies all over the world. The portable, sensitive and cheap devices for rapid detection of these pathogen are in great interest of many research groups. In this paper the description and first results of test of a miniaturized system for detection of common food pathogens by a real-time PCR is presented. The novel fluorescence optical readout instrumentation has been applied. The unique coupling of CCD-based microdetectors array and expertise software enables detection of DNA in ng/ml range. The ral-time PCR of Campylobacter jejuni DNA has been successfully carried out.