DocumentCode
488029
Title
The Modelling and Control of Fractionation Processes for Enzyme and Protein Purification
Author
Niktari, M. ; Richardson, P. ; Ravenhall, R. ; Flanagan, M.T. ; Molloy, J. ; Hoare, M.
Author_Institution
SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, U.K.
fYear
1989
fDate
21-23 June 1989
Firstpage
2436
Lastpage
2440
Abstract
The use of an automated microcentrifuge and flow injection analyser is described for the measurement on-line of the solubility profiles and the resultant fractionation diagram for the purification by salting-out of enzymes from a microbial cell homogenate. The use of this system is demonstrated for the on-line control of the fractional precipitation of alcohol dehydrogenase from a clarified yeast homogenate using ammonium sulphate.
Keywords
Biochemistry; Cells (biology); Control systems; Educational institutions; Fractionation; Particle measurements; Process control; Proteins; Purification; Solvents;
fLanguage
English
Publisher
ieee
Conference_Titel
American Control Conference, 1989
Conference_Location
Pittsburgh, PA, USA
Type
conf
Filename
4790598
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