Construction of Ribavirin Engineering Bacteria

Author

Xing, Chen-Guang ; Chen, Ning ; Xie, Xi-Xian ; Zhao, Xi-Jing ; Xu, Qing-Yang

Author_Institution

Coll. of Biotechnol., Tianjin Univ. of Sci. & Technol., Tianjin, China

Volume

1

fYear

2009

fDate

4-5 July 2009

Firstpage

583

Lastpage

586

Abstract

In this study, the gene encoding purine nucleoside phosphorylase (PNPase) from Bacillus subtilis W168 was identified, cloned and expressed in Bacillus subtilis AG208. The gene encodes a polypeptide of 233 amino acids with a calculated molecular weight of 25,018 Da. The enzyme activity of the recombinant protein (AGPNP) was analyzed by temperature and pH perturbation difference spectra. Results showed that the optimum of temperature was 65degC and the optimum pH was 7.5 and the PNPase activity of the recombinant bacteria was increased by 193.9%.

Keywords

biochemistry; biothermics; drugs; enzymes; genetics; microorganisms; molecular biophysics; proteins; Bacillus subtilis AG208; Bacillus subtilis W168; amino acids; bacteria; enzyme activity; gene encoding; pH effect; polypeptide; protein thermostability; purine nucleoside phosphorylase; recombinant protein; ribavirin; Biochemistry; Chemicals; Cloning; DNA; Electronic mail; Microorganisms; Pharmaceutical technology; Production; Proteins; Temperature; Bacillus subtilis; purine nucleoside phosphorylase; ribavirin;

fLanguage

English

Publisher

ieee

Conference_Titel

Environmental Science and Information Application Technology, 2009. ESIAT 2009. International Conference on

Conference_Location

Wuhan

Print_ISBN

978-0-7695-3682-8

Type

conf

DOI

10.1109/ESIAT.2009.50

Filename

5200189