Photon-counting photobleaching measurements and the effect of dispersion in two-photon microscopy

Author

Field, Jeffrey J. ; Carriles, Ramón ; Squier, Jeff A.

Author_Institution

Dept. of Phys., Colorado Sch. of Mines, Golden, CO, USA

fYear

2009

fDate

2-4 June 2009

Firstpage

1

Lastpage

2

Abstract

The effect of dispersion of the excitation pulse in two-photon microscopy is examined with a photon-counting microscope in both stationary and scanning modalities, and it is demonstrated that transform-limited pulses provide the best signal yield despite increased bleaching rates.

Keywords

bio-optics; biological techniques; diffraction gratings; optical dispersion; optical microscopy; optical prisms; optical pulse shaping; optical saturable absorption; photon counting; two-photon processes; biological imaging; biologically relevant fluorophore; dispersion effect; grism-based laser pulse shaper; photobleaching measurement; photon counting microscope; transform-limited pulse; two-photon microscopy; Biomedical optical imaging; Bleaching; Dispersion; Fluorescence; Optical microscopy; Optical pulse shaping; Optical scattering; Photobleaching; Pulse measurements; Pulse shaping methods; (170.6280) Spectroscopy, fluorescence and luminescence; (180.2520) Fluorescence Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on

Conference_Location

Baltimore, MD

Print_ISBN

978-1-55752-869-8

Electronic_ISBN

978-1-55752-869-8

Type

conf

Filename

5225726