A femtosecond Yb:KGd(WO4)2 laser for biological imaging of yellow fluorescent protein

Author

Sandkuijl, D. ; Major, A. ; Cisek, R. ; Grang, G. ; Mathur, J. ; Stewart, B. ; Barzda, V.

Author_Institution

Dept. of Phys., Univ. of Toronto, Mississauga, ON, Canada

fYear

2009

fDate

2-4 June 2009

Firstpage

1

Lastpage

2

Abstract

Real time multiphoton excitation fluorescence microscopy of enhanced yellow fluorescent protein was performed with low photobleaching using a femtosecond Yb:KGd(WO₄)₂ laser. Using a conventional Ti:S laser this was previously unfeasible due to low excitation efficiency.

Keywords

biological techniques; fluorescence; gadolinium compounds; high-speed optical techniques; laser beam applications; molecular biophysics; multiphoton processes; optical microscopy; optical saturable absorption; potassium compounds; proteins; solid lasers; ytterbium; KGd(WO₄)₂:Yb; biological imaging; excitation efficiency; femtosecond laser; multiphoton excitation fluorescence microscopy; photobleaching; yellow fluorescent protein; Biomedical optical imaging; Fluorescence; Laser excitation; Nonlinear optics; Optical harmonic generation; Optical imaging; Optical microscopy; Optical pumping; Photobleaching; Ultrafast optics; (140.3615) Lasers, Ytterbiums; (180.4315) Nonlinear microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on

Conference_Location

Baltimore, MD

Print_ISBN

978-1-55752-869-8

Electronic_ISBN

978-1-55752-869-8

Type

conf

Filename

5225995