Title :
Intracellular manipulation using nonlinear excitation
Author :
Watanabe, Wataru
Author_Institution :
Photonics Res. Inst., Nat. Inst. of Adv. Ind. Sci. & Technol., Ikeda, Japan
fDate :
Oct. 30 2008-Nov. 2 2008
Abstract :
Nonlinear microscopy using near-infrared femtosecond laser pulses has potential applications in imaging and manipulation of intracellular organelles. I present intracellular surgery, selective tracking, and FRAP analysis of organelles in living cells using femtosecond lasers.
Keywords :
biological techniques; cellular biophysics; fluorescence spectroscopy; high-speed optical techniques; optical microscopy; fluorescence recovery after photobleaching; intracellular manipulation; intracellular organelle imaging; intracellular organelle manipulation; near infrared femtosecond laser pulse; nonlinear excitation; nonlinear microscopy; organelle FRAP analysis; organelle intracellular surgery; organelle selective tracking; Cells (biology); Fluorescence; Laser ablation; Laser excitation; Microscopy; Nonlinear optics; Optical imaging; Optical pulses; Optical scattering; Ultrafast optics;
Conference_Titel :
Optical Fiber Communication & Optoelectronic Exposition & Conference, 2008. AOE 2008. Asia
Conference_Location :
Shanghai
Print_ISBN :
978-1-55752-863-6
Electronic_ISBN :
978-1-55752-863-6
