In-vivo measurement of Indocyanine green biodistribution in mammalian organs using fiber based system

Indocyanine green (ICG) is a fluorescent probe widely used in recent years, and it is also the fluorescent dye that can be clinical used, in both imaging and treatment. So it is important to study its biodistribution and metabolism in mammalian organs, but the accuracy and sampling speed is limited by the traditional in-vitro methods. Now we present a design of an in-vivo multi-channel fluorescence intensity measurement system and an algorism of data processing, to achieve the accurate measurement of fluorescence intensity, continuous sampling, real time monitoring and curve fitting. This system design is based on customized fiber bundles and the principle of reflective fluorescence microscopy. We also present a mouse experiment using this system to study the Indocyanine green (ICG) biodistribution in small mammalian liver, in order to demonstrate the potential applications of this system and also present a new experiment method in the study of dye biodistribution and metabolism.