Title :
Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function
Author :
Thompson, Michael A. ; Lew, Matthew D. ; Badieirostami, Majid ; Moerner, W.E.
Author_Institution :
Depts. of Chem., Stanford Univ., Stanford, CA, USA
Abstract :
Single fluorescent emitters can be localized and tracked in 3D with less than 1000 detected photons by using a DH-PSF. The tracking of quantum dots both in solution and inside a living cell is demonstrated.
Keywords :
fluorescence; optical images; optical microscopy; optical transfer function; quantum dots; detected photons; double-helix point spread function; fluorescence microscopy; fluorescent emitters; quantum dots tracking; single emitter localization; single emitter tracking; Biomedical optical imaging; Cells (biology); Fluorescence; Image resolution; Optical imaging; Optical microscopy; Optical noise; Quantum dots; Signal resolution; Stimulated emission;
Conference_Titel :
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-890-2
Electronic_ISBN :
978-1-55752-890-2
