• DocumentCode
    520118
  • Title

    Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function

  • Author

    Thompson, Michael A. ; Lew, Matthew D. ; Badieirostami, Majid ; Moerner, W.E.

  • Author_Institution
    Depts. of Chem., Stanford Univ., Stanford, CA, USA
  • fYear
    2010
  • fDate
    16-21 May 2010
  • Firstpage
    1
  • Lastpage
    2
  • Abstract
    Single fluorescent emitters can be localized and tracked in 3D with less than 1000 detected photons by using a DH-PSF. The tracking of quantum dots both in solution and inside a living cell is demonstrated.
  • Keywords
    fluorescence; optical images; optical microscopy; optical transfer function; quantum dots; detected photons; double-helix point spread function; fluorescence microscopy; fluorescent emitters; quantum dots tracking; single emitter localization; single emitter tracking; Biomedical optical imaging; Cells (biology); Fluorescence; Image resolution; Optical imaging; Optical microscopy; Optical noise; Quantum dots; Signal resolution; Stimulated emission;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
  • Conference_Location
    San Jose, CA
  • Print_ISBN
    978-1-55752-890-2
  • Electronic_ISBN
    978-1-55752-890-2
  • Type

    conf

  • Filename
    5499680
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=520118