Studying single cells with fiber back-scattering confocal micro-spectrum

Author

Wang, Cheng ; Ma, Junling ; Guo, Xudong

Author_Institution

Sch. of Med. Instrum. & Food Eng., Univ. of Shanghai for Sci. & Technol., Shanghai, China

Volume

4

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

1603

Lastpage

1606

Abstract

Based on fiber confocal technology, combined with cell light scattering theory, fiber confocal back scattering (FCBS) spectrometer, a micro-spectral measurement setup, has been established. It is able to provide micro-medical spectral information. Adherent monolayer human normal gastric epithelium line GES-1 and carcinoma cell line NCI-N87 were measured by the setup and these spectral illuminated that light intensity from NCI-N87 cells was stronger than that from GES-1 in 500nm 3090583800nm range and that GES-1´s scattering spectrum showed a regular intensity change but NCI-N87´s didn´t. This indicated that FCBS spectrometer established can distinguish normal from dyplastic cell at cellular level.

Keywords

backscatter; bio-optics; biological techniques; biomedical measurement; cellular biophysics; FCBS spectrometer; GES-1 cell line; NCI-N87 cell line; carcinoma cell line; cell light scattering theory; fiber back scattering confocal microspectrum; human normal gastric epithelium line; microspectral measurement; wavelength 500 nm to 800 nm; Biomedical optical imaging; Cells (biology); Humans; Optical fibers; Optical scattering; Cell analysis; Scattering; Spectroscopy; Tissue diagnosis;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5639282

Filename

5639282