Title :
Biotransformation of digoxigenin by Arthrobacter simplex TCCC 11037
Author :
Liu, Zengbing ; Sun, Hua ; Zheng, Yu ; He, Chaojun ; Fu, Yuwan ; Wang, Min
Author_Institution :
Key Lab. of Ind. Microbiol., Minist. of Educ., Tianjin, China
Abstract :
Biotransformation of natural products has great potential for production of new drugs or Lead Compounds. In this paper biotransformation of digoxigenin (1) by Arthrobacter simplex TCCC 11037 was investigated. The biotransformation reaction was carried out in a 7-day fermentation process. Three products purified from broth culture supernatants by silica gel column chromatography. And they were identified as digoxigenone (2), 16(17)-ene-digoxigenone (3), and 1(2),16(17)-dien- digoxigenone (4) by FT-ICR-MS and NMR. This is the first report of the production of compound 2 and compound 3 by a biotransformation reaction.
Keywords :
Fourier transform spectra; biochemistry; biotechnology; chemical reactions; chromatography; drugs; fermentation; mass spectra; microorganisms; molecular biophysics; nuclear magnetic resonance; organic compounds; 1(2),16(17)-dien- digoxigenone; 16(17)-ene-digoxigenone; Arthrobacter simplex TCCC 11037; FT-ICR-MS; NMR; biotransformation reaction; culture supernatants; digoxigenin biotransformation; fermentation process; silica gel column chromatography; time 7 day; Carbon; Chemicals; Drugs; Production; Purification; Silicon compounds; Arthrobacter simplex; biotransformation; digoxigenin; digoxin;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
DOI :
10.1109/BMEI.2010.5639441
