Autonomous operating process for zebrafish embryo injection

Author

Wang Yiliao ; Sun Mingzhu ; Zhao Xin ; Zhao Baoquan

Author_Institution

Inst. of Robot. & Autom. Inf. Syst., Nankai Univ., Tianjin, China

fYear

2012

fDate

Aug. 29 2012-Sept. 1 2012

Firstpage

65

Lastpage

70

Abstract

Embryo injection is a significant technique in molecule biology and drug discovery for zebrafish, one of the most popular model organisms. Traditional zebrafish embyo injection is manually conducted under microscope, requiring challenging coordination of hand, eye and foot and their respective precise control as well as ceaseless labour. An autonomous operating process for zebrafish embryo injection is proposed in this paper. Based on a micromanipulation robot system, the operating process integrates computer vision and micromanipulation robot control to realize batch embryo injection automatically. Successful rate of 93% and survival ratio of 89.5% at operating speed of 6-7 embryos are obtained, verifying the feasibility and effectiveness of the process. Finally, the process is applied to investigate the toxicity of a dye and receives success.

Keywords

cellular biophysics; drugs; dyes; eye; medical robotics; micromanipulators; robot vision; toxicology; zoology; autonomous operating process; computer vision; drug discovery; dye; eye; foot; hand; micromanipulation robot system; molecule biology; toxicity; zebrafish embryo injection; Embryo; Fluids; Microinjection; Microscopy; Robot kinematics; Autonomous Operating Process; Visual feedback; Zebrafish Embryo Injection;

fLanguage

English

Publisher

ieee

Conference_Titel

Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2012 International Conference on

Conference_Location

Shaanxi

Print_ISBN

978-1-4673-4588-0

Electronic_ISBN

978-1-4673-4589-7

Type

conf

DOI

10.1109/3M-NANO.2012.6472927

Filename

6472927