Effect of liquids on AFM imaging

Author

Yang Liu ; Zuobin Wang ; Yingmin Qu ; Yinbao Lei ; Yanqin Peng ; Pantao Zhang ; Jinyun Liu ; Wei Jiang ; Yandong Bai

Author_Institution

JR3CN & CNM Centers, Changchun Univ. of Sci. & Technol., Changchun, China

fYear

2012

fDate

Aug. 29 2012-Sept. 1 2012

Firstpage

14

Lastpage

17

Abstract

AFM (atomic force microscope) imaging in liquids is very important in measurement science for bio-applications. This paper discusses the effect of liquids on AFM imaging. In a developed AFM system, a standard 2D grating was used for the study of AFM imaging in different liquids including deionzed water, DMEM (Dulbecco´s Modified Eagle Medium) and DMEM with 10% of FBS (Fetal Bovine Serum). AFM images acquired from different liquids were analyzed in terms of the image contrast and standard deviation of the grating period by Matlab. The experimental results have shown clearly the different effects of liquids on AFM imaging. The AFM imaging quality in liquids is a function of different forces acting on the cantilever and is directly related to the physical properties of the liquids.

Keywords

atomic force microscopy; AFM imaging; DMEM; Dulbecco modified eagle medium; Matlab; atomic force microscope imaging; bio-applications; cantilever; deionzed water; image contrast; liquid effect; measurement science; physical properties; standard 2D grating; standard deviation; Force; Gratings; Liquids; Microscopy; Standards; Viscosity; AFM imaging in liquids; Liquid AFM; atomic force microscope;

fLanguage

English

Publisher

ieee

Conference_Titel

Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2012 International Conference on

Conference_Location

Shaanxi

Print_ISBN

978-1-4673-4588-0

Electronic_ISBN

978-1-4673-4589-7

Type

conf

DOI

10.1109/3M-NANO.2012.6473002

Filename

6473002